At the JGI--or Joint Genome Institute--I had the opportunity to experience one of the largest sacred desoxyribonucleic acid sequencing operations in the world. Basically, I got to see primary the machinery and technology involved in the sour called whole-genome shotgun sequencing, whereby the bless of fts in an organisms genome is determined. One of the hinderance guides, before the tour, gave us an lively voice of this process. In this genome-determining-process, deoxyribonucleic acid from the organism to be sequenced is depleted up into pieces ab expose 2,000 to 4,000 bases long through a process called shearing. Then, these fragments of deoxyribonucleic acid are inserted into the deoxyribonucleic acid of a bacteria. When grown on an nutrient agar shell, only the transformed bacteria with the plasmid deoxyribonucleic acid containing the fragmented desoxyribonucleic acid with genes for ampicillin resistance and the lacZ gene cut off by the inserted DNA can grow on the plate and be sportsmanlike. These face cloth colonies are the ones used in the upcoming steps. Basically, the plasmid DNA is taken out of the bacterium; the fragments are replicated and the terminal base of each fragment is phiz with a fluorescent dye. The tagged fragments are loaded into a high-speed capillary cataphoresis machine, and lasers can read the dyes of the bases as they move through the machine. A computer--at the end--solves the puzzle and determines the organisms genome sequence.
While at the JGI, on our tour, we cut an electroporation machine, which induces the bacteria to open their pores, cause the plasmids to enter into th! e bacteria. Also, we saw a robotic bacteria picker, which picks specific white bacteria colonies out of the plate. Furthermore, we saw a large form of thermal cyclers and sequencing machines. Overall, the tour was worthwhile because we learned how specialized institutions much(prenominal) as the JGI sequence an organisms DNA and had the... If you want to get a full essay, order it on our website: OrderCustomPaper.com
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